B7H4 Recombinant Rabbit Monoclonal Antibody [PSH03-65]
cat.: HA722024
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH03-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human B7H4 aa 101-150 / 282. |
| Positive control: | MDA-MB-468 cell lysate, NIH:OVCAR-3 cell lysate, mouse uterus tissue lysate, rat ovary tissue lysate, SK-Br-3. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q7Z7D3 Human | Q7TSP5 Mouse |
| Alternative names: | B7 family member, H4 B7 H4 B7 homolog 4 B7 superfamily member 1 B7 superfamily, member 1 B7-H4 B7h.5 B7h4 B7S1 B7x BC032925 Immune costimulatory protein B7-H4 Immune costimulatory protein B7H4 MGC41287 PRO1291 Protein B7S1 RP11 229A19.4 T cell costimulatory molecule B7x T-cell costimulatory molecule B7x V set domain-containing T cell activation inhibitor 1 V-set domain-containing T-cell activation inhibitor 1 VCTN1 Vtcn1 VTCN1_HUMAN |
Images
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Fig1:
Western blot analysis of B7H4 on different lysates with Rabbit anti-B7H4 antibody (HA722024) at 1/1,000 dilution. Lane 1: MDA-MB-468 cell lysate (no heat) (15 µg/Lane) Lane 2: HeLa cell lysate (negative) (30 µg/Lane) Lane 3: NIH:OVCAR-3 cell lysate (30 µg/Lane) Lane 4: Mouse uterus tissue lysate (30 µg/Lane) Lane 5: Rat ovary tissue lysate (15 µg/Lane) Predicted band size: 31 kDa Observed band size: 60-75 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722024) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of SK-Br-3 (positive) and HeLa (negative) labeling B7H4 with Rabbit anti-B7H4 antibody (HA722024) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7H4 antibody (HA722024) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.