SP110 Recombinant Rabbit Monoclonal Antibody [PSH03-66]
cat.: HA722025
| Product Type: | Recombinant Rabbit monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH03-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human SP110 aa 301-550 / 689. |
| Positive control: | Jurkat cell lysate, HeLa cell lysate, HL-60 cell lysate, SW480 cell lysate, Daudi cell lysate, K-562 cell lysate, PC-3M cell lysate, human lymph node tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:2,000 |
| Uniprot #: | SwissProt: Q9HB58 Human |
| Alternative names: | FLJ22835 IFI 41 IFI 75 IFI41 IFI75 Interferon induced protein 41 30kD Interferon induced protein 41 Interferon induced protein 41/75 Interferon induced protein 75 52kD Interferon induced protein 75 Interferon-induced protein 41/75 IPR 1 IPR1 Phosphoprotein 41 Phosphoprotein 75 SP 110 SP110 Sp110 nuclear body protein SP110_HUMAN Speckled 110 kDa Transcriptional coactivator Sp110 VODI |
Images
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Fig1:
Western blot analysis of SP110 on different lysates with Rabbit anti-SP110 antibody (HA722025) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HeLa cell lysate Lane 3: HL-60 cell lysate Lane 4: SW480 cell lysate Lane 5: Daudi cell lysate Lane 6: K-562 cell lysate Lane 7: PC-3M cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 78 kDa Observed band size: 29-78 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722025) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SP110 antibody (HA722025) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722025) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.