CBX2 Recombinant Rabbit Monoclonal Antibody [JE34-45]
cat.: HA722027
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE34-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CBX2 aa 433-532 / 532. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse heart tissue lysate, HeLa. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q14781 Human | P30658 Mouse Entrez Gene: 303730 Rat |
| Alternative names: | Cbx 2 CBX2 CBX2_HUMAN CDC A6 CDCA6 Cell division cycle associated 6 Cell division cycle associated6 chromobox homolog 2 (Pc class homolog, Drosophila) Chromobox homolog 2 Chromobox homolog2 Chromobox protein homolog 2 hromobox homolog 2 M33 M33 polycomb like MOD 2 MOD2 Modifier 3 Modifier3 Pc class homolog Polycomb SRXY5 |
Images
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Fig1:
Western blot analysis of CBX2 on different lysates with Rabbit anti-CBX2 antibody (HA722027) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: Mouse heart tissue lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722027) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling CBX2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722027, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.