Raptor Recombinant Rabbit Monoclonal Antibody [JE59-39]
cat.: HA722028
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE59-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 149 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Raptor aa 1,286-1,335 / 1,335. |
| Positive control: | 293T cell lysate, K-562 cell lysate, Neuro-2a cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue. |
| Subcellular location: | Lysosome membrane, Cytoplasm, Cytoplasmic granule. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:500 |
| Uniprot #: | SwissProt: Q8N122 Human | Q8K4Q0 Mouse Entrez Gene: 287871 Rat |
| Alternative names: | KIAA1303 KOG1 Mip1 P150 target of rapamycin (TOR) scaffold protein p150 target of rapamycin (TOR) scaffold protein containing WD repeats P150 target of rapamycin (TOR)-scaffold protein Raptor Regulatory associated protein of mTOR Regulatory associated protein of MTOR complex 1 Regulatory-associated protein of mTOR RPTOR RPTOR_HUMAN |
Images
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Fig1:
Western blot analysis of Raptor on different lysates with Rabbit anti-Raptor antibody (HA722028) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: K-562 cell lysate (30 µg/Lane) Lane 3: Neuro-2a cell lysate (30 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722028) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Raptor antibody (HA722028) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722028) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.