MYCBP2 Recombinant Rabbit Monoclonal Antibody [PSH03-68]
cat.: HA722030
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: PSH03-68
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 514 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MYCBP2 aa 1-300.
Positive control: HeLa cell lysate, THP-1 cell lysate, HUVEC cell lysate, HEK-293 cell lysate, A431 cell lysate, mouse brain tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, HeLa, human brain tissue.
Subcellular location: Nucleus, Cell projection, axon, Cytoplasm, cytoskeleton.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:2,000
1:100
1:500
1:1,000
Uniprot #: SwissProt: O75592 Human | Q7TPH6 Mouse
Entrez Gene: 290447 Rat
Alternative names: AU023734 AW107953 AW546647 C130061D10Rik DKFZp686M08244 FLJ10106 FLJ13826 FLJ21597 FLJ21646 KIAA0916 MYC binding protein 2 PAM Pam, highwire, rpm 1 Pam/highwire/rpm-1 protein Phr1 Protein associated with Myc R75243
Images
HA722030_1.jpg Fig1: Western blot analysis of MYCBP2 on different lysates with Rabbit anti-MYCBP2 antibody (HA722030) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: HUVEC cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: A431 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Mouse spleen tissue lysate (40 µg/Lane)
Lane 8: Rat brain tissue lysate (40 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 514 kDa
Observed band size: 514 kDa

Exposure time: 1 minute;

3-8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722030) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722030_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling MYCBP2 with Rabbit anti-MYCBP2 antibody (HA722030) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MYCBP2 antibody (HA722030) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722030_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MYCBP2 antibody (HA722030) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722030) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722030_4.jpg Fig4: Flow cytometric analysis of HeLa cells labeling MYCBP2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722030, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.