HMGB3 Recombinant Rabbit Monoclonal Antibody [JE63-39]
cat.: HA722033
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HMG4 aa 50-150. |
| Positive control: | HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, Jurkat cell lysate. |
| Subcellular location: | Nucleus, Chromosome, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O15347 Human |
| Alternative names: | chromosomal protein, Nonhistone, HMG4 High mobility group (nonhistone chromosomal) protein 4 High mobility group box 3 High mobility group protein 2a High mobility group protein 4 High mobility group protein B3 High mobility group protein HMG4 HMG 4 HMG-2a HMG-4 HMG2A HMGB 3 Hmgb3 HMGB3_HUMAN MGC90319 Non histone chromosomal protein Nonhistone chromosomal protein HMG4 |
Images
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Fig1:
Western blot analysis of HMGB3 on different lysates with Rabbit anti-HMGB3 antibody (HA722033) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722033) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.