Phospho-SYK (Y525 + Y526) Recombinant Rabbit Monoclonal Antibody [PSH03-70]
cat.: HA722034
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH03-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr525 and 526 of Human SYK. |
| Positive control: | Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes. |
| Subcellular location: | Cell membrane, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P43405 Human |
| Alternative names: | EC 2.7.10.2 kinase Syk KSYK KSYK_HUMAN p72-Syk p72syk Spleen tyrosine kinase Syk Tyrosine protein kinase SYK Tyrosine-protein kinase SYK |
Images
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Fig1:
Western blot analysis of Phospho-SYK (Y525 + Y526) on different lysates with Rabbit anti-Phospho-SYK (Y525 + Y526) antibody (HA722034) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-2 (left): 1 minute 2 seconds; Lane 1-2 (right): 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722034) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes labeling Phospho-SYK (Y525 + Y526) with Rabbit anti-Phospho-SYK (Y525 + Y526) antibody (HA722034) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-SYK (Y525 + Y526) antibody (HA722034) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.