Human HGF Recombinant Rabbit Monoclonal Antibody [PSH03-79]
cat.: HA722043
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HGF aa 32-728 (P14210). |
| Positive control: | Recombinant Human HGF protein (HA210739) |
| Subcellular location: | Extracellular region, Extracellular space. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-78] to Human HGF (Capture) (HA722042) and recombinant standard Human HGF (HA210739). The reference range value is 61.7-5000 pg/ml. |
| Uniprot #: | SwissProt: P14210 Human |
| Alternative names: | DFNB39 F TCF Fibroblast derived tumor cytotoxic factor Hepatocyte growth factor (hepapoietin A; scatter factor) Hepatocyte growth factor Hepatocyte growth factor beta chain Hepatocyte growth factor precursor Hepatopoietin A Hepatopoietin-A Hgf HGF_HUMAN HGFB HPTA Lung fibroblast derived mitogen OTTHUMP00000161349 OTTHUMP00000206710 OTTHUMP00000206711 OTTHUMP00000206712 OTTHUMP00000206713 OTTHUMP00000206730 Scatter factor SF |
Images
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Fig1:
Sandwich ELISA analysis of Human HGF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA722042 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human HGF protein (HA210739) starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722043-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of Human HGF were interpolated from the HGF standard curves and corrected for sample dilution.The mean HGF concentration was determined to be 1,141 pg/ml in THP-1 cell supernatant. |
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Fig3: The concentrations of Human HGF were interpolated from the HGF standard curves and corrected for sample dilution.The mean HGF concentration was determined to be 1,657 pg/ml in mixed human serum from twenty volunteers. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.