IL-13R alpha 2 Recombinant Rabbit Monoclonal Antibody [PSH03-85]
cat.: HA722051
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH03-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL-13R alpha 2 aa 1-350 / 380. |
| Positive control: | A375 cell lysate, U-87 MG cell lysates, human testis tissue, A375. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q14627 Human |
| Alternative names: | Cancer/testis antigen 19 CD213a2 CT19 I13R2_HUMAN IL-13 receptor subunit alpha-2 IL-13R subunit alpha-2 IL-13R-alpha-2 IL-13RA2 IL13 receptor IL13BP IL13R Il13ra2 Interleukin 13 binding protein Interleukin 13 receptor alpha 2 chain Interleukin-13 receptor subunit alpha-2 Interleukin-13-binding protein |
Images
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Fig1:
Western blot analysis of IL-13R alpha 2 on different lysates with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/2,000 dilution. Lane 1: A375 cell lysate (no heat) Lane 2: U-937 cell lysate (no heat) (negative) Lane 3: Daudi cell lysate (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 50-80 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722051) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IL-13R alpha 2 on U-87 MG cell lysates with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 50-70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722051) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722051) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of A375 (positive) and U-937 (negative) cells labeling IL-13R alpha 2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722051, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.