GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH03-87]
cat.: HA722053
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH03-87 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 108 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GABA B Receptor 1 aa 142-591 / 961. |
| Positive control: | Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse cerebellum tissue lysate, rat cerebellum tissue lysate, mouse cerebellum tissue, human cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cell membrane, Postsynaptic cell membrane, Cell projection, dendrite; Secreted. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:5,000 1:50-1:200 1:1,000-1:20,000 |
| Uniprot #: | SwissProt: Q9UBS5 Human | Q9WV18 Mouse | Q9Z0U4 Rat |
| Alternative names: | dJ271M21.1.1 dJ271M21.1.2 FLJ92613 GABA-B receptor 1 GABA-B-R1 GABA-BR1 GABAB R1 GABAB subunit 1c GABABR1 GABBR1 3 GABBR1 GABR1_HUMAN Gamma aminobutyric acid (GABA) B receptor 1 Gamma-aminobutyric acid type B receptor subunit 1 Gb1 GPRC3A Seven transmembrane helix receptor |
Images
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Fig1:
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate (hot lysis) Lane 3: Rat brain tissue lysate (no heat) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) Lane 5: Rat cerebellum tissue lysate Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722053, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/5,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane) Lane 5: Rat cerebellum tissue lysate (20 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722053) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722053) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722053) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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