PTN Recombinant Rabbit Monoclonal Antibody [JE47-40]
cat.: HA722055
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE47-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PTN aa 1-100. |
| Positive control: | Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:3,000 |
| Uniprot #: | SwissProt: P21246 Human | P63089 Mouse | P63090 Rat |
| Alternative names: | HARP HB-GAM HBBM HBGAM HBGF-8 HBGF8 HBNF HBNF-1 HBNF1 heparin affin regulatory protein Heparin binding growth associated molecule Heparin binding growth factor 8 Heparin binding neurite outgrowth promoting factor 1 Heparin-binding brain mitogen Heparin-binding growth factor 8 Heparin-binding growth-associated molecule heparin-binding neurite outgrowth promoting factor Heparin-binding neurite outgrowth-promoting factor 1 NEGF1 Neurite growth promoting factor 1 Neurite outgrowth-promoting factor, heparin-binding OSF-1 OSF1 Osteoblast-specific factor 1 pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) Pleiotrophin PTN PTN_HUMAN |
Images
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Fig1:
Western blot analysis of PTN on different lysates with Rabbit anti-PTN antibody (HA722055) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 3 minutes 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722055) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PTN antibody (HA722055) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722055) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.