BNIP3L Recombinant Rabbit Monoclonal Antibody [JE59-98]
cat.: HA722056
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE59-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BNIP3L aa 120-219 / 219. |
| Positive control: | 293T cell lysate, HeLa cell lysate, MCF7 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, human bone marrow tissue, human kidney tissue, human thyroid cancer tissue, mouse adipose tissue. |
| Subcellular location: | Nucleus envelope, Endoplasmic reticulum, Mitochondrion outer membrane, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: O60238 Human | Q9Z2F7 Mouse Entrez Gene: 140923 Rat |
| Alternative names: | Adenovirus E1B19k binding protein B5 Adenovirus E1B19K-binding protein B5 BCL2/adenovirus E1B 19 kd protein interacting protein 3a BCL2/adenovirus E1B 19 kDa protein interacting protein 3A BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A BCL2/adenovirus E1B 19kDa interacting protein 3 like BNI3L_HUMAN BNIP3a BNIP3H BNIP3L BNIP3L protein NIP3 like protein X NIP3-like protein X NIP3L NIX |
Images
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Fig1:
Western blot analysis of BNIP3L on different lysates with Rabbit anti-BNIP3L antibody (HA722056) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 37 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722056) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of BNIP3L on different lysates with Rabbit anti-BNIP3L antibody (HA722056) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 37 kDa Exposure time: 5 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-BNIP3L antibody (HA722056) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722056) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BNIP3L antibody (HA722056) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722056) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Rabbit anti-BNIP3L antibody (HA722056) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722056) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse adipose tissue with Rabbit anti-BNIP3L antibody (HA722056) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722056) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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