Parathyroid Hormone Recombinant Rabbit Monoclonal Antibody [JE66-58]
cat.: HA722059
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE66-58 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Parathyroid Hormone aa 26-75 / 115. |
| Positive control: | HEK-293 cell lysate, CAL-62 cell lysate, A549 cell lysate, Jurkat cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, human parathyroid tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1;1,000 1:100 |
| Uniprot #: | SwissProt: P01270 Human | P04089 Rat Entrez Gene: 19226 Mouse |
| Alternative names: | hPTH Parathormone Parathyrin Parathyroid hormone 1 Parathyroid hormone Prepro PTH Preproparathyroid hormone PTH PTH1 PTH1 receptor PTH1R PTHR PTHR1 PTHY_HUMAN |
Images
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Fig1:
Western blot analysis of Parathyroid Hormone on different lysates with Rabbit anti-Parathyroid Hormone antibody (HA722059) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: CAL-62 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Lane 6: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 13 kDa Observed band size: 16 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722059) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human parathyroid tissue with Rabbit anti-Parathyroid Hormone antibody (HA722059) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722059) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.