Cathepsin L Recombinant Rabbit Monoclonal Antibody [PSH03-91]
cat.: HA722063
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH03-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cathepsin L aa 84-333 / 333. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, human kidney tissue lysate, human liver tissue lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, COS-1 cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, A549. |
| Subcellular location: | Lysosome, Apical cell membrane, Cytoplasmic vesicle, secretory vesicle, chromaffin granule, Secreted, extracellular space. |
| Recommended Dilutions:
WB IHC-P FC |
1:5,000 1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P07711 Human | P06797 Mouse | P07154 Rat |
| Alternative names: | Cathepsin L cathepsin L, 1 b Cathepsin L1 Cathepsin L1 light chain CathepsinL CATL CATL1_HUMAN cb15 CTSL CTSL1 ctsl1b FLJ31037 hgg1 Major excreted protein MEP MGC123162 wu:fb30g09 |
Images
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Fig1:
Western blot analysis of Cathepsin L on different lysates with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution. Lane 1: A549 cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: HEK-293 cell lysate (15 µg/Lane) Lane 4: MDA-MB-231 cell lysate (15 µg/Lane) Lane 5: Human kidney tissue lysate (30 µg/Lane) Lane 6: Human liver tissue lysate (30 µg/Lane) Lane 7: NIH/3T3 cell lysate (15 µg/Lane) Lane 8: PC-12 cell lysate (15 µg/Lane) Lane 9: Mouse kidney tissue lysate (30 µg/Lane) Lane 10: Rat kidney tissue lysate (30 µg/Lane) Lane 11: Mouse liver tissue lysate (30 µg/Lane) Lane 12: Rat liver tissue lysate (30 µg/Lane) Lane 13: COS-1 cell lysate (15 µg/Lane) Predicted band size: 38 kDa Observed band size: 20-45 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722063) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722063) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722063) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722063) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of A549 cells labeling Cathepsin L. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722063, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Western blot analysis of Cathepsin L on different lysates with Rabbit anti-Cathepsin L antibody (HA722063) at 1/5,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Cathepsin L cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 20 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722063) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.