Phospho-DNA PKcs (S2056) Recombinant Rabbit Monoclonal Antibody [JE41-92]
cat.: HA722066
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE41-92 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 469 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues surrounding Ser2056 of human DNA-PKcs protein. |
| Positive control: | HT-29 treated with UV for 3 hours cell lysate, HT-29 cells treated with UV for 3 hours. |
| Subcellular location: | Nucleus, nucleolus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P78527 Human |
| Alternative names: | DNA dependent protein kinase catalytic subunit DNA PK catalytic subunit DNA-dependent protein kinase catalytic subunit DNA-PK catalytic subunit DNA-PKcs DNAPK DNAPK catalytic subunit DNPK 1 DNPK1 Hyper radiosensitivity of murine scid mutation, complementing 1 Hyperradiosensitivity complementing 1, mouse, homolog of 1 HYRC 1 HYRC HYRC1 IMD26 p350 p460 PKRDC PRKDC PRKDC_HUMAN Protein Kinase DNA Activated Catalytic Polypeptide XRCC 7 XRCC7 |
Images
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Fig1:
Western blot analysis of Phospho-DNA PKcs (S2056) on different lysates with Rabbit anti-Phospho-DNA PKcs (S2056) antibody (HA722066) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with UV for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 469 kDa Observed band size: 469 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722066) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HT-29 cells treated with or without UV for 3 hours labeling Phospho-DNA PKcs (S2056) with Rabbit anti-Phospho-DNA PKcs (S2056) antibody (HA722066) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-DNA PKcs (S2056) antibody (HA722066) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.