Phospho-PKR (T451) Recombinant Rabbit Monoclonal Antibody [JE46-70]
cat.: HA722067
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE46-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues surrounding Thr45 of human PKR protein. |
| Positive control: | HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, HeLa cells treated with 10ng/mL IFN-α1 for 18 hours then add 100nM Calyculin A for 15 minutes. |
| Subcellular location: | Cytoplasm, Nucleus, perinuclear region. |
| Recommended Dilutions:
WB FC IF-Cell |
1:1,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: P19525 Human |
| Alternative names: | Double stranded RNA activated protein kinase; E2AK2_HUMAN eIF-2A protein kinase 2 EIF2AK1 EIF2AK2 Eukaryotic translation initiation factor 2 alpha kinase 2 Eukaryotic translation initiation factor 2-alpha kinase 2 HGNC:9437 Interferon induced double stranded RNA activated protein kinase Interferon inducible elF2 alpha kinase Interferon inducible RNA dependent protein kinase Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase MGC126524 P1/eIF-2A protein kinase P1/eIF2A protein kinase p68 kinase PKR PPP1R83 PRKR Protein kinase interferon inducible double stranded RNA dependent Protein kinase RNA activated Protein kinase RNA-activated Protein phosphatase 1 regulatory subunit 83 Serine/threonine protein kinase TIK Tyrosine protein kinase EIF2AK2 |
Images
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Fig1:
Western blot analysis of Phospho-PKR (T451) on different lysates with Rabbit anti-Phospho-PKR (T451) antibody (HA722067) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate Lane 3: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 70 kDa Exposure time: 3 minutes 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722067) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HeLa cells treated with 10ng/mL IFN-α1 for 18 hours then add 100nM Calyculin A for 15 minutes labeling Phospho-PKR (T451). Cells were fixed and permeabilized. Then stained with the primary antibody (HA722067, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.