TPOR Recombinant Rabbit Monoclonal Antibody [JE58-00]
cat.: HA722068
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-00 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TPOR aa 586-635 / 635. |
| Positive control: | Mouse placenta tissue lysate, NIH/3T3 cell lysate, A431 cell lysate, rat spleen tissue lysate, mouse spleen tissue lysate, A431. |
| Subcellular location: | Cell membrane, Golgi apparatus, Cell surface. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P40238 Human | Q08351 Mouse |
| Alternative names: | C MPL CD110 mpl MPLV Myeloproliferative leukemia protein Myeloproliferative leukemia virus oncogene Proto-oncogene c-Mpl THCYT2 Thrombopoietin receptor TPO R TPO-R TPOR TPOR_HUMAN |
Images
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Fig1:
Western blot analysis of TPOR on different lysates with Rabbit anti-TPOR antibody (HA722068) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (no heat) (20 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of A431 cells labeling TPOR. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722068, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.