Sortilin Recombinant Rabbit Monoclonal Antibody [JE60-00]
cat.: HA722070
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Sortilin aa 601-800 / 831. |
| Positive control: | SK-MEL-28 cell lysate, human lung tissue lysate, human kidney tissue lysate, human liver tissue lysate, SH-SY5Y cell lysate, human brain tissue lysate, human brain tissue. |
| Subcellular location: | Golgi apparatus, Golgi stack membrane, Endosome membrane, Endoplasmic reticulum membrane, Nucleus membrane, Cell membrane, Lysosome membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q99523 Human |
| Alternative names: | 100 kDa NT receptor Glycoprotein 95 Gp 95 Gp95 LDLCQ6 Neurotensin receptor 3 NT 3 NT3 NTR 3 NTR3 OTTHUMP00000013784 SORT 1 SORT_HUMAN SORT1 (gene name) Sort1 Sortilin 1 Sortilin |
Images
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Fig1:
Western blot analysis of Sortilin on different lysates with Rabbit anti-Sortilin antibody (HA722070) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: Human lung tissue lysate (40 µg/Lane) Lane 3: Human kidney tissue lysate (40 µg/Lane) Lane 4: Human liver tissue lysate (40 µg/Lane) Lane 5: SH-SY5Y cell lysate (20 µg/Lane) Lane 6: Human brain tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 100 kDa Exposure time: Lane 1-4: 1 minute 7 seconds; Lane 5-6: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Sortilin antibody (HA722070) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722070) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.