ACO1 Recombinant Rabbit Monoclonal Antibody [JE63-98]
cat.: HA722071
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE63-98 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ACO1 aa 1-50 / 889. |
| Positive control: | HeLa cell lysate, 293T cell lysate, HCT 116 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, HeLa. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB FC |
1:2,000-1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P21399 Human | P28271 Mouse | Q63270 Rat |
| Alternative names: | ACO 1 ACO1 ACOC_HUMAN Aconitase 1 soluble Aconitase Aconitase1 Aconitate hydratase ACONS Citrate hydro lyase Citrate hydro-lyase Cytoplasmic aconitate hydratase Ferritin repressor protein IRE BP 1 IRE-BP 1 IREB 1 IREB1 IREBP IREBP1 Iron regulatory protein 1 Iron responsive element binding protein 1 Iron-responsive element-binding protein 1 IRP 1 IRP1 OTTHUMP00000045233 |
Images
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Fig1:
Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (HA722071) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722071) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ACO1 on different lysates with Rabbit anti-ACO1 antibody (HA722071) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ACO1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722071) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling ACO1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722071, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.