SNX4 Recombinant Rabbit Monoclonal Antibody [JE65-39]
cat.: HA722072
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SNX4 aa 351-400 / 450. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Early endosome membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95219 Human | Q91YJ2 Mouse Entrez Gene: 360725 Rat |
| Alternative names: | ATG24B SNX 4 snx4 SNX4_HUMAN Sorting nexin 4 Sorting nexin-4 |
Images
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Fig1:
Western blot analysis of SNX4 on different lysates with Rabbit anti-SNX4 antibody (HA722072) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52/36 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.