CD11b Recombinant Rabbit Monoclonal Antibody [PSH03-96]
cat.: HA722075
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: PSH03-96
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 127 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD11b aa 1-100.
Positive control: TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, RAW264.7 cell lysate, J774A.1 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, human cervical cancer tissue, human spleen tissue, human tonsil tissue, mouse spleen tissue, rat spleen tissue.
Subcellular location: Cell membrane, Membrane raft.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
1:2,000
1:5,000-1:20,000
1:1,000
Uniprot #: SwissProt: P11215 Human | P05555 Mouse
Entrez Gene: 25021 Rat
Alternative names: antigen CD11b (p170) Antigen CD11b p170 CD11 antigen like family member B CD11 antigen-like family member B CD11b CD11b/CD18 CD49d Cell surface glycoprotein MAC-1 subunit alpha Complement component 3 receptor 3 subunit Complement Component Receptor 3 Alpha Complement receptor type 3, alpha subunit CR 3 alpha chain (CR3A) CR 3 alpha chain CR-3 alpha chain CR3 CR3A F730045J24Rik Integrin Alpha M Integrin alpha M chain Integrin alpha-M Integrin beta 2 alpha subunit Integrin subunit alpha M integrin, alpha M (complement component 3 receptor 3 subunit) ITAM_HUMAN ITGAM Leukocyte adhesion receptor MO1 Ly-40 MAC 1 Mac-1a MAC1 Mac1, alpha subunit MAC1A Macrophage antigen alpha polypeptide MGC117044 Mo1, alpha subunit MO1A Neutrophil adherence receptor alpha M subunit Neutrophil adherence receptor SLEB6
Images
HA722075_1.jpg Fig1: Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA722075) at 1/2,000 dilution.

Lane 1: TF-1 cell lysate (10 µg/Lane)
Lane 2: THP-1 cell lysate (15 µg/Lane)
Lane 3: U-937 cell lysate (30 µg/Lane)
Lane 4: Jurkat cell lysate (negative) (10 µg/Lane)
Lane 5: RAW264.7 cell lysate (5 µg/Lane)
Lane 6: J774A.1 cell lysate (5 µg/Lane)

Predicted band size: 127 kDa
Observed band size: 170 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722075_2.jpg Fig2: Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA722075) at 1/2,000 dilution.

Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse heart tissue lysate (negative)
Lane 3: Rat spleen tissue lysate
Lane 4: Rat heart tissue lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 127 kDa
Observed band size: 170 kDa

Exposure time: 12 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722075_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-CD11b antibody (HA722075) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722075) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722075_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD11b antibody (HA722075) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722075) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722075_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD11b antibody (HA722075) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722075) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722075_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD11b antibody (HA722075) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722075) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722075_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD11b antibody (HA722075) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722075) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722075_8.jpg Fig8: Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD11b with Rabbit anti-CD11b antibody (HA722075) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722075, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
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