KDM5B Recombinant Rabbit Monoclonal Antibody [PSH03-97]
cat.: HA722076
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH03-97 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 176 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human KDM5B aa 960-1,009 / 1,544. |
| Positive control: | BT-20 cell lysate, MDA-MB-468 cell lysate, AGS cell lysate, A549 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9UGL1 Human |
| Alternative names: | Cancer/testis antigen 31 CT31 Histone demethylase JARID1B JARID1B Jumonji AT rich interactive domain 1B Jumonji/ARID domain-containing protein 1B Kdm5b KDM5B_HUMAN Lysine (K) specific demethylase 5B Lysine demethylase 5B Lysine specific demethylase 5B Lysine-specific demethylase 5B PLU-1 PLU1 PPP1R98 Protein phosphatase 1 regulatory subunit 98 PUT1 putative DNA/chromatin binding motif RBBP2H1A RBP2 like RBP2-H1 Retinoblastoma-binding protein 2 homolog 1 Retinoblastoma-binding protein 2 homolog 1A |
Images
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Fig1:
Western blot analysis of KDM5B on different lysates with Rabbit anti-KDM5B antibody (HA722076) at 1/2,000 dilution. Lane 1: BT-20 cell lysate Lane 2: MDA-MB-468 cell lysate Lane 3: AGS cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 176 kDa Observed band size: 145 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722076) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
KDM5B was immunoprecipitated in 0.2mg A549 cell lysate with HA722076 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722076 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: ER1802-21 IP in A549 cell lysate Lane 3: Rabbit IgG instead of ER1802-21 in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.