beta Amyloid 1-42 Recombinant Rabbit Monoclonal Antibody [PSH02-83]
cat.: HA722079
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-83 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 4 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human beta Amyloid 1-42 peptide. |
| Subcellular location: | Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-99] to Human beta Amyloid 1-42 (Detector) (HA722080). The reference range value is 24.7-2000pg/ml. |
| Uniprot #: | SwissProt: P05067 Human |
| Alternative names: | A4 A4_HUMAN AAA ABETA ABPP AICD-50 AICD-57 AICD-59 AID(50) AID(57) AID(59) Alzheimer disease amyloid protein Amyloid intracellular domain 50 Amyloid intracellular domain 57 Amyloid intracellular domain 59 APP APPI Beta amyloid protein 42 Beta APP42 Beta-APP42 C31 Cerebral vascular amyloid peptide CVAP Gamma-CTF(50) Gamma-CTF(57) Gamma-CTF(59) PN-II PreA4 Protease nexin-II S-APP-alpha S-APP-beta |
Images
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Fig1:
Sandwich ELISA analysis of Human Aβ42 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722079 diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human Aβ42 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody HA722080-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Sandwich ELISA analysis of Human Aβ37, Aβ38, Aβ39, Aβ41, Aβ42 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722079) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Aβ37, Aβ38, Aβ39, Aβ41, Aβ42 protein starting with 2000 pg/ml and detect antibody (HA722080, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig3:
Sandwich ELISA analysis of Human Aβ42 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722079 diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Aβ42 protein starting from 1000 pg/ml to 0 pg/ml and detect antibody HA722080-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.