Phospho-STAT1 (Y701) Recombinant Rabbit Monoclonal Antibody [PSH04-02]
cat.: HA722083
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH04-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr701 of Human STAT1. |
| Positive control: | HeLa treated with 100ng/mL IFNγ for 30 minutes cell lysate, HeLa treated with 50ng/mL IFNα1 for 30 minutes cell lysate, A431 treated with 100ng/mL EGF for 30 minutes cell lysate, HeLa cells treated with 100ng/mL IFNα1 for 5 minutes. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P42224 Human |
| Alternative names: | Signal transducer and activator of transcription 1 91kD CANDF7 DKFZp686B04100 IMD31A IMD31B IMD31C ISGF 3 ISGF-3 OTTHUMP00000163552 OTTHUMP00000165046 OTTHUMP00000165047 OTTHUMP00000205845 Signal transducer and activator of transcription 1 91kDa Signal transducer and activator of transcription 1 Signal transducer and activator of transcription 1, 91kD Signal transducer and activator of transcription 1-alpha/beta STAT 1 Stat1 STAT1_HUMAN STAT91 Transcription factor ISGF 3 components p91 p84 Transcription factor ISGF-3 components p91/p84 Transcription factor ISGF3 components p91/p84 XStat1 |
Images
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Fig1:
Western blot analysis of Phospho-STAT1 (Y701) on different lysates with Rabbit anti-Phospho-STAT1 (Y701) antibody (HA722083) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL IFNγ for 30 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 50ng/mL IFNα1 for 30 minutes cell lysate Lane 5: A431 cell lysate Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 91/84 kDa (STAT1α/β) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722083) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells treated with 100ng/mL IFNα1 for 5 minutes labeling Phospho-STAT1 (Y701) with Rabbit anti-Phospho-STAT1 (Y701) antibody (HA722083) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-STAT1 (Y701) antibody (HA722083) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.