LIAS Recombinant Rabbit Monoclonal Antibody [PSH04-04]
cat.: HA722085
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH04-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LIAS aa 1-372 / 372. |
| Positive control: | K-562 cell lysate, LO2 cell lysate, Raji cell lysate, MCF7 cell lysate, Jurkat cell lysate, HepG2 cell lysate, PC-3M cell lysate, C2C12 cell lysate, L6 cell lysate, mouse liver tissue lysate, mouse colon tissue lysate, mouse brain tissue lysate, rat liver tissue lysate, rat testis tissue lysate, C2C12. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: O43766 Human | Q99M04 Mouse | Q5XIH4 Rat |
| Alternative names: | HUSSY 01 LAS LIP 1 Lip syn LIP1 Lipoate synthase Lipoic acid synthetase Lipoic acid synthetase mitochondrial Lipoyl synthase, mitochondrial LS MGC23245 |
Images
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Fig1:
Western blot analysis of LIAS on different lysates with Rabbit anti-LIAS antibody (HA722085) at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: LO2 cell lysate (20 µg/Lane) Lane 3: Raji cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: HepG2 cell lysate (20 µg/Lane) Lane 7: PC-3M cell lysate (20 µg/Lane) Lane 8: C2C12 cell lysate (20 µg/Lane) Lane 9: L6 cell lysate (20 µg/Lane) Lane 10: Mouse liver tissue lysate (40 µg/Lane) Lane 11: Mouse colon tissue lysate (40 µg/Lane) Lane 12: Mouse brain tissue lysate (40 µg/Lane) Lane 13: Rat liver tissue lysate (40 µg/Lane) Lane 14: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 39 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722085) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of C2C12 cells labeling LIAS with Rabbit anti-LIAS antibody (HA722085) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIAS antibody (HA722085) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.