HA722089

ABL1 Recombinant Rabbit Monoclonal Antibody [PSH04-08]

cat.: HA722089

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Monoclonal
Clone number:	PSH04-08

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 123 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human ABL1 aa 601-1,000 / 1,130.
Positive control:	K-562 cell lysate, HeLa cell lysate, Daudi cell lysate, THP-1 cell lysate, K-562, human breast cancer tissue, human lung cancer tissue, mouse kidney tissue, HeLa.
Subcellular location:	Cytoplasm, cytoskeleton, Nucleus, Mitochondrion; Nucleus membrane.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:1,000 1:100 1:200 1:1,000
Uniprot #:	SwissProt: P00519 Human \| P00520 Mouse
Alternative names:	Abelson murine leukemia viral oncogene homolog 1 Abelson tyrosine protein kinase 1 Abl 1 ABL ABL proto oncogene 1 non receptor tyrosine kinase ABL1 ABL1_HUMAN bcr/abl bcr/c abl oncogene protein c ABL c abl oncogene 1 non receptor tyrosine kinase c abl oncogene 1 receptor tyrosine kinase c ABL1 JTK7 p150 Proto oncogene tyrosine protein kinase ABL1 Proto-oncogene c-Abl Tyrosine-protein kinase ABL1 v abl Abelson murine leukemia viral oncogene homolog 1 v abl

Images

Fig1: Western blot analysis of ABL1 on different lysates with Rabbit anti-ABL1 antibody (HA722089) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Daudi cell lysate
Lane 4: THP-1 cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 123 kDa
Observed band size: 130/250 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722089) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunocytochemistry analysis of K-562 cells labeling ABL1 with Rabbit anti-ABL1 antibody (HA722089) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ABL1 antibody (HA722089) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

	Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-ABL1 antibody (HA722089) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722089) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ABL1 antibody (HA722089) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722089) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ABL1 antibody (HA722089) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722089) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Flow cytometric analysis of HeLa cells labeling ABL1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722089, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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