Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH04-09 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 55 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human FBXO3 aa 1-450 / 471. |
Positive control: | MDA-MB-231 cell lysate, HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, JAR cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB |
1:2,000 |
Uniprot #: | SwissProt: Q9UK99 Human | Q9DC63 Mouse |
Alternative names: | F Box G - Domain 3 F box only protein 3 F box protein 3 F box protein FBX3 F-box only protein 3 FBA FBX3 FBX3_HUMAN FBXO3 |
Fig1:
Western blot analysis of FBXO3 on different lysates with Rabbit anti-FBXO3 antibody (HA722090) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MCF7 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: JAR cell lysate Lane 7: HepG2 cell lysate Lane 8: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/48 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722090) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |