FBXO3 Recombinant Rabbit Monoclonal Antibody [PSH04-09]
cat.: HA722090
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: PSH04-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Recombinant protein within human FBXO3 aa 1-450 / 471.
Positive control: MDA-MB-231 cell lysate, HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, JAR cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q9UK99 Human | Q9DC63 Mouse
Alternative names: F Box G - Domain 3 F box only protein 3 F box protein 3 F box protein FBX3 F-box only protein 3 FBA FBX3 FBX3_HUMAN FBXO3
Images
HA722090_1.jpg Fig1: Western blot analysis of FBXO3 on different lysates with Rabbit anti-FBXO3 antibody (HA722090) at 1/2,000 dilution.

Lane 1: MDA-MB-231 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: JAR cell lysate
Lane 7: HepG2 cell lysate
Lane 8: NIH/3T3 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 55 kDa
Observed band size: 55/48 kDa

Exposure time: 1 minute 18 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722090) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.