FBXO3 Recombinant Rabbit Monoclonal Antibody [PSH04-09]
cat.: HA722090
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH04-09 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FBXO3 aa 1-450 / 471. |
| Positive control: | MDA-MB-231 cell lysate, HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, JAR cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9UK99 Human | Q9DC63 Mouse |
| Alternative names: | F Box G - Domain 3 F box only protein 3 F box protein 3 F box protein FBX3 F-box only protein 3 FBA FBX3 FBX3_HUMAN FBXO3 |
Images
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Fig1:
Western blot analysis of FBXO3 on different lysates with Rabbit anti-FBXO3 antibody (HA722090) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MCF7 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: JAR cell lysate Lane 7: HepG2 cell lysate Lane 8: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/48 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722090) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.