Mouse PD1 Recombinant Rabbit Monoclonal Antibody [PSH04-18]
cat.: HA722101
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH04-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31.8 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse PD1 aa 25-167 (Q02242). |
| Positive control: | Recombinant standard Mouse PD1 protein (HA210625). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-17] to Mouse PD1 (Capture) (HA722100) and recombinant standard Mouse PD1 protein (HA210625). The reference range value is 12.3-3000pg/ml. |
| Uniprot #: | SwissProt: Q02242 Mouse |
| Alternative names: | CD279 CD279 antigen hPD 1 hPD l hPD-1 hSLE1 PD 1 PD-1 PD1 PDCD 1 PDCD1 PDCD1_HUMAN Programmed cell death 1 Programmed cell death 1 protein Programmed cell death protein 1 Protein PD 1 Protein PD-1 SLEB2 Systemic lupus erythematosus susceptibility 2 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse PD-1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722100) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse PD-1 protein (HA210625) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722101) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of PD-1 were interpolated from the PD-1 standard curve and corrected for sample dilution. The mean PD-1 concentration was determined to be 141 pg/ml in mouse spleen tissue extract, 231 pg/ml in mouse thymus tissue extract. There was no detectable signal in NIH/3T3 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.