Human MMP-9 Recombinant Rabbit Monoclonal Antibody [PSH04-24]
cat.: HA722107
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH04-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MMP9 aa 20-707 (P14780). |
| Positive control: | Recombinant Human MMP9 protein (HA210698). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-23] to Human MMP9 (Capture) (HA722106) and recombinant standard Human MMP9 protein (HA210698). The reference range value is 20.6-5000 pg/ml. |
| Uniprot #: | SwissProt: P14780 Human |
| Alternative names: | 82 kDa matrix metalloproteinase-9 92 kDa gelatinase 92 kDa type IV collagenase CLG 4B CLG4B Collagenase Type 4 beta Collagenase type IV 92 KD EC 3.4.24.35 Gelatinase 92 KD Gelatinase B Gelatinase beta GelatinaseB GELB Macrophage gelatinase MANDP2 Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) Matrix Metalloproteinase 9 MMP 9 MMP-9 MMP9 MMP9_HUMAN Type V collagenase |
Images
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Fig1:
Sandwich ELISA analysis of Human MMP9 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722106) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP9 protein (HA210698) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722107) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-one volunteers 0.5%. The mean MMP9 concentration was determined to be 397.2 ng/ml in mixed serum from twenty-one volunteers. |
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Fig3: The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are human urine 100%. The mean MMP9 concentration was determined to be 1624.2 pg/ml in human urine. |
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Fig4: U-2 OS cells were stimulated with 200nM TPA or vehicle control in McCoy's 5a+10%FBS medium and incubated for 48 hours.The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution.Undiluted samples are TPA stimulated U-2 OS cell extract 2.5% and unstimulated U-2 OS cell extract 2.5%. The mean MMP9 concentration was determined to be 44.3 ng/ml in TPA stimulated U-2 OS cell extract and undetectable in the unstimulated U-2 OS control. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.