Human CRP Recombinant Rabbit Monoclonal Antibody [PSH04-25]
cat.: HA722108
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH04-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25.0 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CRP aa 19-224 (P02741). |
| Positive control: | Recombinant Human CRP protein (HA210648). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-26] to Human CRP (Detector) (HA722109) and recombinant standard Human CRP protein (HA210648). The reference range value is 617-50000 pg/ml. |
| Uniprot #: | SwissProt: P02741 Human |
| Alternative names: | Pentraxin 1, short C reactive protein C reactive protein pentraxin related C-reactive protein(1-205) CRP CRP_HUMAN MGC88244 Pentraxin 1 PTX 1 PTX1 |
Images
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Fig1:
Sandwich ELISA analysis of Human CRP matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722108) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CRP protein (HA210648) starting from 50,000 pg/ml to 0 pg/ml and detect antibody (HA722109) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2: The concentrations of CRP were interpolated from the CRP standard curves and corrected for sample dilution.Undiluted sample is serum 1: 200,000. The mean CRP concentration was determined to be 85,871 ng/ml in mixed serum from twenty-four volunteers. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.