EIF2S1 Recombinant Rabbit Monoclonal Antibody [PSH04-29]
cat.: HA722112
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH04-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EIF2S1 aa 1-315 / 315. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, A549 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, HepG2, human breast tissue, mouse breast tissue, rat breast tissue. |
| Subcellular location: | Cytoplasm, Stress granule. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:100 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P05198 Human | Q6ZWX6 Mouse | P68101 Rat |
| Alternative names: | EIF 2 alpha EIF 2 EIF 2A EIF 2alpha eIF-2-alpha eIF-2A EIF-2alpha EIF2 alpha EIF2 EIF2A EIF2S1 Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa Eukaryotic translation initiation factor 2 subunit 1 alpha Eukaryotic translation initiation factor 2 subunit 1 Eukaryotic translation initiation factor 2 subunit alpha IF2A_HUMAN |
Images
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Fig1:
Western blot analysis of EIF2S1 on different lysates with Rabbit anti-EIF2S1 antibody (HA722112) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lane 5: A549 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat kidney tissue lysate Lane 11: Rat spleen tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722112) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling EIF2S1 with Rabbit anti-EIF2S1 antibody (HA722112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EIF2S1 antibody (HA722112) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-EIF2S1 antibody (HA722112) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse breast tissue with Rabbit anti-EIF2S1 antibody (HA722112) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-EIF2S1 antibody (HA722112) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of HepG2 cells labeling EIF2S1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722112, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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