XIAP Recombinant Rabbit Monoclonal Antibody [PSH04-30]
cat.: HA722113
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human XIAP aa 301-450 / 497. |
| Positive control: | 293T cell lysate, MCF7 cell lysate, HT-29 cell lysate, A549 cell lysate, HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human breast cancer tissue, human colon tissue, rat colon tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50 |
| Uniprot #: | SwissProt: P98170 Human | Q60989 Mouse | Q9R0I6 Rat |
| Alternative names: | AP 13 API3 Apoptosis Inhibitor 3 Baculoviral IAP repeat containing 4 Baculoviral IAP Repeat Containing Protein 4 Baculoviral IAP repeat-containing protein 4 BIRC 4 BIRC4 E3 ubiquitin-protein ligase XIAP hIAP-3 hIAP3 HILP IAP 3 IAP like protein IAP-3 IAP-like protein IAP3 ILP 1 ILP ILP1 Inhibitor of apoptosis protein 3 Inhibitor of Apoptosis X Linked Mammalian IAP Homologue A MIHA X linked IAP X linked inhibitor of apoptosis X linked inhibitor of apoptosis E3 ubiquitin protein ligase X linked inhibitor of apoptosis protein X-linked IAP x-linked inhibitor of apopotosis proteins X-linked inhibitor of apoptosis protein Xiap XIAP_HUMAN XLP2 |
Images
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Fig1:
Western blot analysis of XIAP on different lysates with Rabbit anti-XIAP antibody (HA722113) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: HT-29 cell lysate Lane 4: A549 cell lysate Lane 5: HeLa cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Mouse brain tissue lysate Lane 10: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 50 kDa Exposure time: Lane 1-10 (left): 20 seconds; Lane 1-10 (right): 3 minutes; ECL: K1801;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722113) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of XIAP with Rabbit anti-XIAP antibody (HA722113) at 1/2,000 dilution. Lane 1: Wild-type Caco-2 whole cell lysate . Lane 2/3: XIAP knockdown Caco-2 whole cell lysate. Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801;4-20% SDS-PAGE gel. HA722113 was shown to specifically react with XIAP in wild-type Caco-2 cells. Weakened bands were observed when XIAP knockdown samples were tested. Wild-type and XIAP knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA722113) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-XIAP antibody (HA722113) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722113) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-XIAP antibody (HA722113) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722113) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-XIAP antibody (HA722113) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722113) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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