MARCO Recombinant Rabbit Monoclonal Antibody [PSH04-31]
cat.: HA722114
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MARCO aa 401-450 / 520. |
| Positive control: | Human liver tissue, human spleen tissue, mouse spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-P |
1:1,000 |
| Uniprot #: | SwissProt: Q9UEW3 Human | Q60754 Mouse |
| Alternative names: | AI323439 Ly112 Macrophage receptor MARCO Macrophage receptor with collagenous structure Marco MARCO_HUMAN SCARA2 Scavenger receptor class A member 2 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MARCO antibody (HA722114) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722114) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MARCO antibody (HA722114) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722114) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-MARCO antibody (HA722114) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722114) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.