GALC Recombinant Rabbit Monoclonal Antibody [PSH04-32]
cat.: HA722115
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GALC aa 1-500 / 685. |
| Positive control: | 293T cell lysate, A375 cell lysate, A549 cell lysate, SH-SY5Y cell lysate, HaCaT cell lysate, human brain tissue, human liver cancer tissue. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P54803 Human | P54818 Mouse Entrez Gene: 314360 Rat |
| Alternative names: | Gacy Galactocerebrosidase Galactocerebroside beta galactosidase Galactocerebroside beta-galactosidase Galactosylceramidase Galactosylceramide beta galactosidase Galactosylceramide beta-galactosidase galactosylceraminidase GALC GALC_HUMAN GALCERase Twitcher |
Images
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Fig1:
Western blot analysis of GALC on different lysates with Rabbit anti-GALC antibody (HA722115) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: A375 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Predicted band size: 77 kDa Observed band size: 48 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722115) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GALC antibody (HA722115) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722115) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-GALC antibody (HA722115) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722115) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.