Clusterin Recombinant Rabbit Monoclonal Antibody [PSH04-33]
cat.: HA722116
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH04-33 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Clusterin aa 400-449 / 449. |
| Positive control: | HeLa cell lysate, SK-Br-3 cell lysate, A431 cell lysate, PANC-1 cell lysate, MCF7 cell lysate, T-47D cell lysate, human lung cancer tissue, human prostate cancer tissue, human pancreas tissue, human tonsil tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Mitochondrion membrane, cytosol, Microsome, Endoplasmic reticulum, Mitochondrion, perinuclear region, Cytoplasmic vesicle, secretory vesicle, chromaffin granule. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000 1:8,000 1:2,000 |
| Uniprot #: | SwissProt: P10909 Human |
| Alternative names: | 40 AAG 4 AAG4 Aging associated protein 4 Aging-associated gene 4 protein AI893575 APO J Apo-J APOJ ApoJalpha ApoJbeta Apolipoprotein J ApolipoproteinJ CLI CLU CLU1 CLU2 CLUS_HUMAN Clusterin alpha chain Clusterin Clusterin beta chain Complement associated protein SP 40 40 Complement associated protein SP 40 Complement associated protein SP40 Complement cytolysis inhibitor a chain Complement cytolysis inhibitor Complement cytolysis inhibitor b chain Complement lysis inhibitor Complement-associated protein SP-40 D14Ucla3 Dimeric acid glycoprotein Glycoprotein 80 Glycoprotein III GP80 Ku70-binding protein 1 KUB 1 KUB1 MGC24903 NA1/NA2 RATTRPM2B SGP 2 SGP2 SP 40 SP40 Sugp-2 Sulfated glycoprotein 2 Testosterone repressed prostate message 2 Testosterone-repressed prostate message 2 TRPM 2 TRPM-2 TRPM2 TRPM2B Trpmb |
Images
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Fig1:
Western blot analysis of Clusterin on different lysates with Rabbit anti-Clusterin antibody (HA722116) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SK-Br-3 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: PANC-1 cell lysate (20 µg/Lane) Lane 5: MCF7 cell lysate (20 µg/Lane) Lane 6: T-47D cell lysate (20 µg/Lane) Predicted band size: 52 kDa Observed band size: 35-80 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722116) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: IF-Tissue Species: Human Site: pancreas Sample: Paraffin-embedded section Antibody concentration: 1/2,000 |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.