ROR gamma T Recombinant Rabbit Monoclonal Antibody [PSH04-38]
cat.: HA722121
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-38 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ROR gamma T aa 101-300 / 497 (P51449-2). |
| Positive control: | HCT 116 cell lysate, rat thymus tissue lysate, human thymus tissue, rat thymus tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500 |
| Uniprot #: | SwissProt: P51449 Human Entrez Gene: 368158 Rat |
| Alternative names: | IMD42 MGC129539 NR1F3 Nuclear receptor ROR gamma Nuclear receptor ROR-gamma Nuclear receptor RZR gamma Nuclear receptor RZR-gamma Nuclear receptor subfamily 1 group F member 3 RAR related orphan nuclear receptor variant 2 RAR related orphan receptor C RAR related orphan receptor C, isoform a RAR related orphan receptor gamma RAR-related orphan receptor C Retinoic acid binding receptor gamma Retinoid related orphan receptor gamma Retinoid-related orphan receptor-gamma Rorc RORG RORG_HUMAN RZR GAMMA RZRG TOR |
Images
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Fig1:
Western blot analysis of ROR gamma T on different lysates with Rabbit anti-ROR gamma T antibody (HA722121) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 55 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-ROR gamma T antibody (HA722121) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722121) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-ROR gamma T antibody (HA722121) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722121) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.