Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39]
cat.: HA722122
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IHC-P, IHC-Fr, Dot Blot, ELISA, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH04-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | DIG-OVA |
| Recommended Dilutions:
WB IHC-P IHC-Fr Dot Blot ELISA IF-Tissue IP |
1:2,000 1:200-1:5,000 1:50-1:1,000 1:2,000 1:5,000-1:20,000 1:50-1:1,000 1-2μg/sample |
| Alternative names: | 1672-46-4 BRN 0096479 DIG EINECS 216-806-2 HSDB 7108 Lanadigenin Lanadigigenin ST056392 Digoxigenin |
Images
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Fig1:
Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (HA722122) at 1/2,000 dilution. Lane 1: DIG-BSA (2 ng/Lane) Lane 2: BSA (negative) (2 ng/Lane) Lane 3: 293T cell lysate (negative) (20 µg/Lane) Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722122) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxigenin antibody (HA722122) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722122) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (HA722122) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722122) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.