Digoxin Recombinant Rabbit Monoclonal Antibody [PSH04-40]
cat.: HA722123
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Species independent
Applications: WB, ELISA, IHC-P, IHC-Fr, Dot Blot, IF-Tissue, IP
Clonality: Monoclonal
Clone number: PSH04-40
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: DIG-OVA
Recommended Dilutions:
  WB
  ELISA
  IHC-P
  IHC-Fr
  Dot Blot
  IF-Tissue
  IP
1:2,000
1:5,000-1:20,000
1:200-1:5,000
1:50-1:1,000
1:2,000
1:50-1:1,000
1-2μg/sample
Alternative names: 1672-46-4 BRN 0096479 DIG EINECS 216-806-2 HSDB 7108 Lanadigenin Lanadigigenin ST056392 Digoxigenin 地高辛
Images
HA722123_1.jpg Fig1: Western blot analysis of Digoxin on different lysates with Rabbit anti-Digoxin antibody (HA722123) at 1/2,000 dilution.

Lane 1: DIG-BSA (2 ng/Lane)
Lane 2: BSA (negative) (2 ng/Lane)
Lane 3: 293T cell lysate (negative) (20 µg/Lane)

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722123) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722123_2.jpg Fig2: Competitive ELISA analysis of Digoxin / Estradiol / Testoserone was performed by coating wells of a 96-well plate with 50 µl per well of Digoxin-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Digoxin monoclonal antibody at concentration of 1 µg/mL with serial diluted Digoxin / Estradiol / Testoserone starting from a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1/5,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA722123_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxin antibody (HA722123) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722123_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-Digoxin antibody (HA722123) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722123_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat spleen tissue (negative) with Rabbit anti-Digoxin antibody (HA722123) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.