Phospho-BCAR1 (Y410) Recombinant Rabbit Monoclonal Antibody [JE47-97]
cat.: HA722124
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE47-97 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Tyr410 of Human BCAR1. |
| Positive control: | HeLa cell lysate, HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate. |
| Subcellular location: | Cell junction, focal adhesion, Cytoplasm, Cell projection, axon. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P56945 Human |
| Alternative names: | BCAR 1 Bcar1 BCAR1_HUMAN Breast cancer anti estrogen resistance 1 Breast cancer anti estrogen resistance 1 protein Breast cancer anti-estrogen resistance protein 1 CAS Cas scaffolding protein family member 1 CAS1 Cass1 Crk associated substrate Crk associated substrate p130Cas CRK-associated substrate CRKAS FLJ12176 FLJ45059 p130cas |
Images
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Fig1:
Western blot analysis of Phospho-BCAR1 (Y410) on different lysates with Rabbit anti-Phospho-BCAR1 (Y410) antibody (HA722124) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 130 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722124) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.