Phospho-Nucleophosmin (T199) Recombinant Rabbit Monoclonal Antibody [JE63-16]
cat.: HA722125
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-16 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr199 of Human Nucleophosmin. |
| Positive control: | HeLa cell lysate, HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate. |
| Subcellular location: | Nucleus, nucleolus, nucleoplasm, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P06748 Human |
| Alternative names: | B23 MGC104254 NO38 NPM NPM_HUMAN NPM1 Nucleolar phosphoprotein B23 Nucleolar protein NO38 Nucleophosmin (nucleolar phosphoprotein B23 numatrin) Nucleophosmin nucleophosmin nucleoplasmin family member 1 Nucleophosmin/nucleoplasmin family member 1 Numatrin OTTHUMP00000161024 OTTHUMP00000161025 OTTHUMP00000223397 OTTHUMP00000223398 |
Images
|
Fig1:
Western blot analysis of Phospho-Nucleophosmin (T199) on different lysates with Rabbit anti-Phospho-Nucleophosmin (T199) antibody (HA722125) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate Lane 3: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 15 µg/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722125) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.