TIMP3 Recombinant Rabbit Monoclonal Antibody [PSH04-41]
cat.: HA722126
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH04-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TIMP3 aa 1-211 / 211. |
| Positive control: | HepG2 cell lysate, A431 cell lysate, rat placenta tissue lysate, rat spleen tissue lysate, HeLa, HepG2. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P35625 Human | P48032 Rat |
| Alternative names: | HSMRK222 K222 K222TA2 Metalloproteinase inhibitor 3 MIG 5 protein MIG5 protein Protein MIG 5 Protein MIG-5 SFD Sorsby fundus dystrophy pseudoinflammatory TIMP 3 TIMP metallopeptidase inhibitor 3 TIMP-3 TIMP3 TIMP3_HUMAN Tissue Inhibitor of Metalloproteinase 3 Tissue inhibitor of metalloproteinases 3 Tissue inhibitor of metalloproteinases3 |
Images
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Fig1:
Western blot analysis of TIMP3 on different lysates with Rabbit anti-TIMP3 antibody (HA722126) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: A431 cell lysate Lane 3: Human liver tissue lysate (negative) Lane 4: Rat placenta tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 20 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722126) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling TIMP3 with Rabbit anti-TIMP3 antibody (HA722126) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIMP3 antibody (HA722126) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunocytochemistry analysis of HepG2 cells labeling TIMP3 with Rabbit anti-TIMP3 antibody (HA722126) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIMP3 antibody (HA722126) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.