HA722128

TXNDC9 Recombinant Rabbit Monoclonal Antibody [PSH04-43]

cat.: HA722128

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC, IF-Tissue
Clonality:	Monoclonal
Clone number:	PSH04-43

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 27 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human TXNDC9 aa 1-226 / 226.
Positive control:	HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, Raji cell lysate, LNCaP cell lysate, HEK-293 cell lysate, HepG2 cell lysate, HUVEC cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, mouse kidney tissue lysate, rat testis tissue lysate, HeLa, human colon tissue, mouse testis tissue, rat testis tissue, human testis tissue, NIH/3T3.
Subcellular location:	Cytoplasm, Nucleus, cytoskeleton, microtubule organizing center, centrosome, Midbody.
Recommended Dilutions: WB IF-Cell IHC-P FC IF-Tissue	1:2,000 1:100 1:500-1:2,000 1:1,000 1:100-1:400
Uniprot #:	SwissProt: O14530 Human \| Q9CQ79 Mouse \| Q8K581 Rat
Alternative names:	APACD ATP binding protein associated with cell differentiation ATP-binding protein associated with cell differentiation ES cell related protein PHLP3 Phosducin like protein 3 Protein 1 4 Protein 1-4 Thioredoxin domain containing 9 Thioredoxin domain containing protein 9 Thioredoxin domain-containing protein 9 TXND9_HUMAN Txndc9 TXNDC9 protein

Images

Fig1: Western blot analysis of TXNDC9 on different lysates with Rabbit anti-TXNDC9 antibody (HA722128) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Raji cell lysate (20 µg/Lane)
Lane 5: LNCaP cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: HepG2 cell lysate (20 µg/Lane)
Lane 8: HUVEC cell lysate (20 µg/Lane)
Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
Lane 10: Mouse testis tissue lysate (40 µg/Lane)
Lane 11: Mouse kidney tissue lysate (40 µg/Lane)
Lane 12: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: Lane 1-12 (left): 27 seconds; Lane 1-12 (right): 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722128) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TXNDC9 antibody (HA722128) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722128) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TXNDC9 antibody (HA722128) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722128) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TXNDC9 antibody (HA722128) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722128) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunocytochemistry analysis of HeLa cells labeling TXNDC9 with Rabbit anti-TXNDC9 antibody (HA722128) at 1/100 dilution and competitor's antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TXNDC9 antibody (HA722128) at 1/100 dilution and competitor's antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Fig6: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TXNDC9 antibody (HA722128) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722128) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Flow cytometric analysis of NIH/3T3 cells labeling TXNDC9.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722128, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.