SAV1 Recombinant Rabbit Monoclonal Antibody [PSH04-45]
cat.: HA722130
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: PSH04-45
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SAV1 aa 1-383 / 383.
Positive control: HCT 116 cell lysate, HeLa cell lysate, 293T cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, SW480 cell lysate, BxPC-3 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human kidney tissue, human testis tissue, mouse kidney tissue, mouse testis tissue, rat kidney tissue, rat testis tissue, HCT 116.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:2,000
1:200-1:1,000
1:1,000
Uniprot #: SwissProt: Q9H4B6 Human | Q8VEB2 Mouse | A4V8B4 Rat
Alternative names: 1700040G09Rik 45 kDa WW domain protein hWW 45 hWW45 Protein salvador homolog 1 salvador family WW domain containing protein 1 salvador homolog 1 (Drosophila) Salvador homolog 1 Salvador, Drosophila, homolog of SAV 1 SAV SAV1 SAV1_HUMAN WW 45 WW domain containing WW domain-containing adaptor 45 WW domain-containing protein, 45-KD WW45 WW45 protein WWP 4 WWP4
Images
HA722130_1.jpg Fig1: Western blot analysis of SAV1 on different lysates with Rabbit anti-SAV1 antibody (HA722130) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 6: SW480 cell lysate (20 µg/Lane)
Lane 7: BxPC-3 cell lysate (20 µg/Lane)
Lane 8: 786-0 cell lysate (negative) (20 µg/Lane)
Lane 9: A549 cell lysate (20 µg/Lane)
Lane 10: NIH/3T3 cell lysate (20 µg/Lane)
Lane 11: PC-12 cell lysate (20 µg/Lane)
Lane 12: Mouse testis tissue lysate (40 µg/Lane)
Lane 13: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 3 minute4; ECL: Lane 1-13 (left): K1801; Lane 1-13 (right): K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722130) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722130_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SAV1 antibody (HA722130) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722130) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722130_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SAV1 antibody (HA722130) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722130) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722130_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SAV1 antibody (HA722130) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722130) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722130_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SAV1 antibody (HA722130) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722130) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722130_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SAV1 antibody (HA722130) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722130) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722130_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SAV1 antibody (HA722130) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722130) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722130_8.jpg Fig8: Flow cytometric analysis of HCT 116 cells labeling SAV1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722130, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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