Histone H3 (acetyl K9) Recombinant Rabbit Monoclonal Antibody [PSH04-47]
cat.: HA722132
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, IF-Tissue, FC, ChIP, Dot Blot, IP
Clonality: Monoclonal
Clone number: PSH04-47
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 15 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Histone H3 (acetyl K9) aa 1-50.
Positive control: HeLa cell lysate, HeLa treated with 500ng/mL TSA for 4 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate, C6, HeLa cells treated with 500ng/mL TSA for 4 hours, human stomach tissue, mouse colon tissue, rat colon tissue, HeLa.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IF-Tissue
  FC
  ChIP
  Dot Blot
  IP
1:1,000
1:5,000-1:15,000
1:2,000
1:200
1:1,000
Use 0.5~2 μg for 25 μg of chromatin.
1:1,000
1-2μg/sample
Uniprot #: SwissProt: P68431 human | P84243 human | Q16695 human | Q6NXT2 human | Q71DI3 human | P68433 mouse | P84228 mouse | Q6LED0 rat
Alternative names: H3 histone family, member A H3/A H31_HUMAN H3FA Hist1h3a HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J histone 1, H3a Histone cluster 1, H3a Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l H3K9AC
Images
HA722132_1.jpg Fig1: Western blot analysis of Histone H3 (acetyl K9) on different lysates with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722132) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722132_2.jpg Fig2: Immunocytochemistry analysis of C6 cells labeling Histone H3 (acetyl K9) with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/10,000 dilution and competitor's antibody at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/10,000 dilution and competitor's antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722132_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722132) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722132_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722132) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722132_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722132) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722132_6.jpg Fig6: Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling Histone H3 (acetyl K9) with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/15,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/15,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722132_7.jpg Fig7: Flow cytometric analysis of HeLa cells labeling Histone H3 (acetyl K9).

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722132, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA722132_8.jpg Fig8: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H3 (acetyl K9) (HA722132) / Competitor's antibody / Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
HA722132_9.jpg Fig9: Dot blot analysis of Histone H3 (acetyl K9) on different proteins with Rabbit anti-Histone H3 (acetyl K9) antibody (HA722132) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature.

Lane 1: Unmodified Histone H3 (negative)
Lane 2: Mono-Methyl-Histone H3 (Lys9) (negative)
Lane 3: Tri-Methyl-Histone H3 (Lys9) (negative)
Lane 4: Acetyl-Histone H3 (Lys9) (positive)
Lane 5: Acetyl-Histone H3 (Lys4) (negative)
Lane 6: Acetyl-Histone H3 (Lys27) (negative)

Proteins loading: 100ng, 25ng, 5ng;

Blocking and dilution buffer: 5% NFDM/TBST;

Exposure time: 30 seconds; ECL: K1801.
HA722132_10.jpg Fig10: Histone H3 (acetyl K9) was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722132 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722132 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: HA722132 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722132 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 18 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.