P2Y12 Recombinant Rabbit Monoclonal Antibody [PSH04-48]
cat.: HA722133
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH04-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | Human brain tissue, mouse brain tissue, rat brain tissue, mouse cerebrum tissue, mouse hippocampus tissue, rat cerebrum tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-P IHC-Fr IF-Tissue |
1:1,000 1:1,000-1:2000 1:500 |
| Uniprot #: | SwissProt: Q9H244 Human | Q9CPV9 Mouse | Q9EPX4 Rat |
| Alternative names: | ADP glucose receptor ADP-glucose receptor ADPG R ADPG-R ADPGR BDPLT8 G protein coupled receptor SP1999 Gi coupled ADP receptor HORK 3 Gi coupled ADP receptor HORK3 HORK 3 HORK3 P2RY 12 P2RY12 P2T(AC) P2Y 12 P2Y purinoceptor 12 P2Y(12)R P2Y(AC) P2Y(ADP) P2Y(cyc) P2Y12 P2Y12 platelet ADP receptor P2Y12_HUMAN Platelet ADP receptor Purinergic receptor P2RY12 Purinergic receptor P2Y G protein coupled 12 Purinergic receptor P2Y12 Putative G protein coupled receptor SP 1999 SP1999 |
Images
|
Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:2,000 Antigen retrieval: Not required |
|
Fig2:
Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:2,000 Antigen retrieval: Not required |
|
Fig3:
Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:500 |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-P2Y12 antibody (HA722133) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-P2Y12 antibody (HA722133) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-P2Y12 antibody (HA722133) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.