Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Mouse |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH04-51 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 14 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within mouse Ly6c1 aa 51-100 / 131. |
Positive control: | Mouse lung tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P0CW02 Mouse | P0CW03 Mouse |
Alternative names: | Ly 6c Ly6c protein Ly6c1 Ly6c2 Lymphocyte antigen 6 complex Lymphocyte antigen 6 complex locus C Lymphocyte antigen 6C Lymphocyte antigen 6C1 Lymphocyte antigen 6C2 Lymphocyte antigen Ly 6C |
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Fig1:
Western blot analysis of Ly6c on different lysates with Rabbit anti-Ly6c antibody (HA722134) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse testis tissue lysate (low expression) Lane 3: Mouse liver tissue lysate (low expression) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722134) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |