Ly6c Recombinant Rabbit Monoclonal Antibody [PSH04-51]
cat.: HA722134
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: WB
Clonality: Monoclonal
Clone number: PSH04-51
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse Ly6c1 aa 51-100 / 131.
Positive control: Mouse lung tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P0CW02 Mouse | P0CW03 Mouse
Alternative names: Ly 6c Ly6c protein Ly6c1 Ly6c2 Lymphocyte antigen 6 complex Lymphocyte antigen 6 complex locus C Lymphocyte antigen 6C Lymphocyte antigen 6C1 Lymphocyte antigen 6C2 Lymphocyte antigen Ly 6C
Images
HA722134_1.jpg Fig1: Western blot analysis of Ly6c on different lysates with Rabbit anti-Ly6c antibody (HA722134) at 1/1,000 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Mouse testis tissue lysate (low expression)
Lane 3: Mouse liver tissue lysate (low expression)
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse heart tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722134) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.