LIS1 Recombinant Rabbit Monoclonal Antibody [JE37-43]
cat.: HA722143
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE37-43 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LIS1 aa 51-100 / 410. |
| Positive control: | HeLa cell lysate, 293T cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, Nucleus membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P43034 Human | P63005 Mouse | P63004 Rat |
| Alternative names: | LIS 1 LIS 2 LIS-1 LIS1 LIS1_HUMAN LIS2 Lissencephaly 1 protein Lissencephaly-1 protein MDCR MDS PAF acetylhydrolase 45 kDa subunit PAF AH 45 kDa subunit PAF AH alpha PAF-AH 45 kDa subunit PAF-AH alpha PAFAH alpha PAFAH PAFAH1B1 PAFAHA Platelet activating factor acetylhydrolase 1b regulatory subunit 1 Platelet activating factor acetylhydrolase isoform Ib alpha subunit Platelet-activating factor acetylhydrolase IB subunit alpha |
Images
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Fig1:
Western blot analysis of LIS1 on different lysates with Rabbit anti-LIS1 antibody (HA722143) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.