TEAD1 Recombinant Rabbit Monoclonal Antibody [JE45-37]
cat.: HA722144
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE45-37
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human TEF1/TEAD-1 aa 200-300.
Positive control: HeLa cell lysate, A549 cell lysate, 293T cell lysate, SiHa cell lysate, C2C12 cell lysate, L6 cell lysate, human skeletal muscle tissue, human spleen tissue, mouse skeletal muscle tissue, mouse spleen tissue, rat skeletal muscle tissue, rat spleen tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
1:1,000
1:200-1:1,000
Uniprot #: SwissProt: P28347 Human | P30051 Mouse
Entrez Gene: 361630 Rat
Alternative names: AA Atrophia areata peripapillary chorioretinal degeneration NTEF 1 NTEF-1 NTEF1 Protein GT IIC Protein GT-IIC REF 1 REF1 SV40 transcriptional enhancer factor TCF 13 TCF-13 TCF13 TEA domain family member TEA domain family member 1 (SV40 transcriptional enhancer factor) TEA domain family member 1 TEAD 1 TEAD 1 protein TEAD-1 TEAD1 TEAD1 protein TEAD1_HUMAN TEF 1 TEF1 Transcription factor 13 (SV40 transcriptional enhancer factor) Transcription factor 13 Transcriptional enhancer factor 1 Transcriptional enhancer factor TEF-1 Transcriptional enhancer factor TEF1
Images
HA722144_1.jpg Fig1: Western blot analysis of TEAD1 on different lysates with Rabbit anti-TEAD1 antibody (HA722144) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lane 4: SiHa cell lysate
Lane 5: C2C12 cell lysate
Lane 6: L6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 48/50 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722144_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-TEAD1 antibody (HA722144) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722144_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TEAD1 antibody (HA722144) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722144_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-TEAD1 antibody (HA722144) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722144_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TEAD1 antibody (HA722144) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722144_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-TEAD1 antibody (HA722144) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722144_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TEAD1 antibody (HA722144) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722144) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.