Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Recombinant Rabbit Monoclonal Antibody [JE57-42]
cat.: HA722146
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE57-42
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 64/81/75 kDa
Isotype: IgG
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser474 of human PAK4.
Positive control: A549 cell lysate, HeLa cell lysate, MCF7 cell lysate, HUVEC cell lysate, HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate.
Subcellular location: Cytoplasm; Mitochondrion, Cytoplasm, Nucleus; Cytoplasm, Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O96013 Human | Q9P286 Human | Q9NQU5 Human
Alternative names: KIAA1142 p21 activated kinase 4 p21 Cdc42/Rac1-actiated kinase 4 P21 protein (Cdc42/Rac) activated kinase 4 p21(CDKN1A) activated kinase 4 p21-activated kinase 4 PAK 4 PAK-4 Pak4 PAK4_HUMAN Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs Serine threonine kinase PAK 4 Serine/threonine protein kinase PAK 4 Serine/threonine protein kinase PAK4 Serine/threonine-protein kinase PAK 4 EC 2.7.11.1 KIAA1264 MGC26232 p21 activated kinase 7 p21 protein (Cdc42/Rac)-activated kinase 7 p21(CDKN1A) activated kinase 7 p21-activated kinase 5 p21-activated kinase 7 PAK 5 PAK 7 PAK-5 PAK-7 PAK5 PAK7 PAK7_HUMAN Protein kinase PAK5 Serine/threonine protein kinase PAK 7 Serine/threonine-protein kinase PAK 7 CDKN1A activated kinase 6 EC 2.7.1.37 p21 activated protein kinase 6 p21 protein (Cdc42/Rac)-activated kinase 6 p21(CDKN1A) activated kinase 6 p21-activated kinase 6 p21activated kinase 6 PAK 6 PAK-6 Pak......
Images
HA722146_1.jpg Fig1: Western blot analysis of Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) on different lysates with Rabbit anti-Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) antibody (HA722146) at 1/1,000 dilution.

Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: HUVEC cell lysate (10 µg/Lane)
Lane 5: HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate (30 µg/Lane)

Predicted band size: 64/81/75 kDa
Observed band size: 64 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722146) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.