GATA2 Recombinant Rabbit Monoclonal Antibody [PSH04-53]
cat.: HA722152
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH04-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GATA2 aa 1-50. |
| Positive control: | SH-SY5Y cell lysate, LNCaP cell lysate, PC-12 cell lysate, SH-SY5Y. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: P23769 Human | Q924Y4 Rat |
| Alternative names: | DCML Endothelial transcription factor GATA 2 Endothelial transcription factor GATA-2 Endothelial transcription factor GATA2 FLJ45948 GATA 2 GATA binding protein 2 GATA-binding protein 2 Gata2 GATA2_HUMAN IMD21 MGC2306 MONOMAC NFE 1B NFE1B OTTHUMP00000216240 |
Images
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Fig1:
Western blot analysis of GATA2 on different lysates with Rabbit anti-GATA2 antibody (HA722152) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: LNCaP cell lysate Lane 3: Jurkat cell lysate (negative) Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: Lane 1: 9 seconds; Lane 2-4: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722152) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of SH-SY5Y (positive) and Jurkat (negative) labeling GATA2 with Rabbit anti-GATA2 antibody (HA722152) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GATA2 antibody (HA722152) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.